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estimation of trimebutine maleate in tablet dosage form by rp-hplc

Time:2015/7/28 7:08:30

A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Trimebutine Maleate in tablet dosage form. An XTerra(R) C18 analytical column (250 x 4.6 mm, 5 ?μm partical size) with mobile phase consisting of mixture of buffer 0.02M Ammonium Acetate in water and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 275 nm. The retention time was 17.0 minutes. The detector response was linear in the concentration of 20-300 mcg/mL. The respective linear regression equation being y = 1914.1 x -1911. The limit of detection and limit of quantification was 0.5 mcg/mL and 1.5 mcg/mL respectively. The percentage assay of Trimebutine Maleate was 99.8 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Trimebutine Maleate in bulk drug and in its pharmaceutical dosage form.

Effects of Trimebutine Maleate on Electrical Activities of Isolated Mammalian Cardiac Preparations

The effects of trimebutine maleate on electrical activity in guinea-pig isolated papillary muscles and rabbit sino-atrial nodes have been studied by means of a standard microelectrode method. In papillary muscles, trimebutine (above 10 μM) decreased the maximum rate of rise (V?max) and the action potential duration at 90% repolarization (APD90), whereas the resting potential was not significantly altered. As to a decrease in V?max, trimebutine produced a negative shift of the curve relating Vmax to the resting potential along the voltage axis. Trimebutine also depressed the slow action potentials of papillary muscles produced by 27 mM K and 0·2 mitt Ba. In spontaneously beating sino-atrial node preparations, trimebutine (above 10 μ) decreased the heart rate, Vmax and the rate of diastolic depolarization. These results indicate that trimebutine maleate possesses a depressant action on the electrical activities of the fast- and slow-response fibres of the heart mainly due to inhibitions of both fast Na+ and slow Ca2+ channels.